Thin-layer Rapid Use Epicutaneous Test (T.R.U.E. Test®) is a ready-to-use patch test method designed for diagnosis of allergic contact dermatitis.

The standard panel consists of two tape strips, panel 1, with 11 allergen patches and a negative control and panel 2, with 12 allergen patches. T.R.U.E. TestTM panels 1 and 2 contain 23 of the most frequent contact allergens. With the 23 allergens the test currently consists of, it is possible to detect about 60-70% of contact allergic reactions. Thus, there is a need for expanding the number of allergens included in T.R.U.E.Test in order to detect more contact allergic reactions. Bacitracin is one of these allergens, and the purpose of this study is to determine a concentration for the patch by using a bacitracin dilution series.

Inclusion Criteria:

1. Positive Bacitracin patch test within the latest 5 years.
2. Age more or equal to 18 years.
3. Signed informed consent.

Exclusion Criteria:

1. Topical treatment with corticosteroids or immunosuppressives during the latest 7 days on the test area or near the test area.
2. Systemic treatment with corticosteroids or immunosuppressives during the latest 7 days.

4. Treatment with UV-light during the latest 3 weeks. 5. Widespread active dermatitis or dermatitis on test area. 6. Breast-feeding or pregnancy. Females of childbearing potential must demonstrate a negative pregnancy test before inclusion in the study.

7. Subjects not able to cooperate.