The purpose of this Phase 2 clinical research study is to examine the safety of an experimental gene transfer agent, Ad2/HIF-1α/VP16, and its ability to stimulate the growth of new blood vessels from existing blood vessels (a process called angiogenesis) in an attempt to improve the flow of blood in the legs of patients with peripheral arterial disease (PAD). Specifically, this study will enroll patients with severe intermittent claudication (IC) which is the stage of PAD in which a patient’s walking ability is severely limited, causing pain in the legs upon exercise due to inadequate blood flow to the muscles of the lower limbs.

Condition	Intervention	Phase
Intermittent Claudication Peripheral Vascular Disease Atherosclerosis	Gene Transfer: Ad2/HIF-1α/VP16	Phase II

Further Study Details: 

Primary Outcomes: % change in peak walking time at 6 months using standardized treadmill walking test
Secondary Outcomes: Peak walking time at 3 months, 1 year; Claudication onset time at 3 months, 6 months, 1 year; Quality of life questionnaires at 3 months, 6 months, 1 year; Resting ankle brachial index at 3 months, 6 months, 1 year
Expected Total Enrollment:  300

This Phase 2 gene transfer study will look at whether different doses of Ad2/HIF-1α/VP16 can be tolerated safely by direct injection into the leg muscles where the blood flow is not sufficient to meet the oxygen demands of the leg muscles. The study will also assess whether patients who receive the investigational drug product are able to increase their maximal walking time using a standardized treadmill walking test.

The study design is a randomized, double-blind, placebo-controlled, parallel group, multi-center, Phase 2 dose-selection study. Seventy-five patients will be enrolled into each of 4 study drug groups (3 groups of Ad2/HIF-1α/VP16 gene transfer and 1 placebo group) for a total of 300 patients overall. Three different doses of Ad2/HIF-1α/VP16 gene transfer will be studied. The dose range was previously tested in animals and in the Phase 1 human studies. A placebo group is included in the study to compare safety and efficacy of different doses of Ad2/HIF-1α/VP16 with placebo. Each patient will receive a single set of 20 injections (100 μL each) of gene transfer or placebo in one administration to each leg for a total of 40 injections.

Ages Eligible for Study:  40 Years   -   80 Years,  Genders Eligible for Study:  Both

Criteria

Inclusion Criteria:

• Males and females 40 to 80 years of age, inclusive.
• Clinical diagnosis of PAD, secondary to atherosclerosis, in both lower limbs, confirmed by objective evidence: 1. An ankle-brachial index (ABI) of ≤ 0.90 at rest in at least 1 lower limb 2. The ABI after exercise must be reduced by ≥ 20% from the ABI at rest in the index leg (the most symptomatic leg during the treadmill testing). The post-exercise ABI will also be performed on the other leg if the resting ABI > 0.90. This post-exercise ABI in the non-index leg must be reduced by ≥ 20% for the patient to qualify. 3. If the ABI cannot be measured in either leg (due to non-compressible arteries), then a toe-brachial index (TBI) of ≤ 0.70 may be used in its place to confirm PAD.
• Symptoms of severe intermittent claudication (IC) in at least 1 lower limb persisting for ≥ 6 months
• Patients with a peak walking time (PWT) of 1 to 10 minutes (inclusive) using the standardized exercise treadmill test at each of the 2 consecutive treadmill tests performed at least a week apart during the Screening period. The mean PWT from the 2 consecutive visits must also be 1 to 10 minutes (inclusive).
• During Screening, patients must demonstrate consistency of PWTs between 2 standardized exercise treadmill tests (Walk 1 and Walk 2) performed at least 1 week apart.
• Consistency of the PWT between the 2 visits is achieved if the difference between PWT at Walk 1 and Walk 2 is ≤ 25% of the higher of the 2 PWTs ([higher PWT - lower PWT]/higher PWT).
• If the difference between PWT at Walk 1 and Walk 2 is > 25% of the higher of the 2 PWTs, a third treadmill test (Walk 3) may be performed at the discretion of the Principal Investigator between 7 and 14 days following Walk 2. The variability in PWT warranting the performance of Walk 3 must be secondary to circumstances that may contribute to the observed variation (e.g., prior exertion, inconsistent timing, ingestion of a meal within 4 hours, etc). To qualify for the study, the difference between PWT at Walk 2 and Walk 3 must be ≤ 25% of the higher of the 2 PWTs ([higher PWT - lower PWT]/higher PWT).
• An acceptable mean PWT must be achieved within 4 weeks of treatment administration.
• Patients have been considered for other potential treatment options including exercise rehabilitation, smoking cessation, and pharmacological therapy prior to enrollment.
• Claudication severity, concomitant medications for the treatment of CAD, PAD, and IC, smoking status and exercise habits should be clinically stable for 3 months prior to Screening.
• Patients who are committed to following the protocol requirements as evidenced by written informed consent.

Exclusion Criteria:

• Patients with Critical Limb Ischemia (CLI). (NOTE: Rutherford Category 4: ischemic rest pain, Rutherford Category 5: non-healing ischemic ulcers and minor tissue loss, Rutherford Category 6: non-healing ischemic ulcers and major tissue loss)
• Patients in whom arterial insufficiency in the lower extremity is the result of acute limb ischemia or an immunological or inflammatory non-atherosclerotic disorder (eg, thromboangiitis obliterans [Buerger’s Disease]) and systemic sclerosis [both limited and diffuse forms]).
• A PAD-specific surgical revascularization procedure within 6 months of enrollment or a percutaneous procedure within the previous 3 months, or patients likely to require a revascularization procedure within 6 months after enrollment.
• Patients with aortoiliac disease that limits inflow in either leg: 1. Patients with concomitant aortoiliac disease (i.e., patients with a significant component of inflow disease in the distal aorta, common or external iliac, or proximal common femoral artery) as assessed by an imaging modality (e.g., segmental limb pressures and waveform analysis, duplex ultrasound scanning, magnetic resonance angiography, or radio-contrast arteriogram) performed within 1 year prior to Screening. If subject has had a bypass after the imaging study, then documentation of graft patency is required within 6 months prior to randomization. 2. If it is suspected at Screening that a patient has aortoiliac disease based on vascular examination, an imaging modality (e.g., segmental limb pressures and waveform analysis, duplex ultrasound scanning, magnetic resonance angiography, or radio-contrast arteriogram) must be performed to rule it out if there is not one available within the times specified above.
• Patients in whom walking impairment due to pain in the index leg is the result of these nonatherosclerotic comorbid conditions: venous claudication, chronic compartment syndrome, peripheral nerve pain (e.g., severe peripheral neuropathy), pseudoclaudication caused by spinal cord compression, or acute limb ischemia which, in the Principal Investigator’s judgment are severe enough to confound the assessment of the patient’s IC.
• Conditions other than IC of significant severity that could confound PWT on the standardized exercise treadmill test causing premature or inconsistent termination of exercise (e.g., angina pectoris, heart failure [New York Heart Association {NYHA} Classes III and IV], respiratory disease [e.g., chronic obstructive pulmonary disease], orthopedic disease, neurological disorders, rheumatologic disorders [e.g., severe degenerative joint diseases], dyspnea, fatigue, prior lower limb amputation, including amputations proximal to the metatarsal or phalangeal joints).
• Presence or history of cancer within 5 years or not current with recommended screening guidelines for colorectal, lung, prostate, breast, cervical, and uterine cancers, with the exception of low grade and fully resolved non-melanoma skin malignancy.
• Patients with a well-defined clinical or genetic disorder predisposing to malignancy should be excluded (e.g., von Hippel Lindau, familial polyposis coli, BRCA1, BRCA2, etc).
• Patients with baseline funduscopic evidence of active proliferative diabetic retinopathy, preproliferative diabetic retinopathy, or wet AMD AND/OR Patients with a history of treatment for active proliferative diabetic retinopathy or wet AMD within 5 years of study participation.
• Diabetes type 1 (juvenile onset)
• Poorly controlled type 2 diabetes (ie, HbA1C >10%) at Screening
• Active hepatitis defined as clinically significant increase in liver enzymes (ie, 3 times the ULN) or other current infectious disease
• Patients with symptoms of respiratory infection at time of Screening and/or randomization period and/or patients who have been on systemic or oral antibiotics for active infection within 2 weeks of study drug administration.
• Patients with clinically significant abnormal hematology (eg, hematocrit < 30%, white blood cell count > 10,000), blood chemistry, renal, hepatic, or other laboratory parameters that could be the result of an underlying malignancy or systemic infection (e.g., serum creatinine  2.5 mg/dL), as judged by the investigator.
• Patients with the following comorbidities who may not be healthy enough to successfully complete all protocol requirements or in whom results may be particularly difficult to assess: 1. Concurrent severe congestive heart failure (NYHA Classes III and IV), 2. Life-threatening ventricular arrhythmias, unstable angina (characterized by increasingly frequent episodes with modest exertion or at rest, worsening severity, and prolonged duration), and/or myocardial infarction within 4 weeks 3. Coronary artery bypass grafting or percutaneous coronary intervention within 3 months 4. Transient ischemic attack within 3 months 5. Deep vein thrombosis within 3 months 6. Severe chronic obstructive pulmonary disease (room air arterial PO2 < 60 mmHg or PCO2 > 50 mmHg, or abnormal pulmonary function tests (FEV1 < 1.2 L/sec) 7. Thrombocytopenia 8. Undergoing hemodialysis 9. Patients with immunocompromised conditions, organ transplant recipients and/or need for immunosuppressive therapy 10. Neurological dementia (i.e., Alzheimer’s Disease) 11. Hemorrhagic stroke
• Patients with a known allergy to the vehicle, placebo control, or any other medications or imaging agents required for participation in this study.
• Fertile women who are pregnant, nursing, or using either no or an inadequate form of contraception.
• Fertile men who are not willing to use barrier-type contraception.
• Patients with a recent history of alcoholism or drug abuse, or severe emotional, behavioral or psychiatric problems, who may not be able to adequately comply with the requirements of the study.
• Patients receiving experimental medications or participating in another study using an experimental drug or experimental procedure within 30 days of screening into this study.
• Patients previously enrolled in a prior angiogenic gene therapy clinical study, unless patient was a known placebo patient.

Please refer to this study by ClinicalTrials.gov identifier  NCT00117650

Alabama
      Baptist Medical Center Princeton, Birmingham,  Alabama,  35211,  United States; Recruiting
California
      VA Palo Alto Health Care System, Palo Alto,  California,  94304,  United States; Recruiting
      Stanford University School of Medicine, Stanford,  California,  94305,  United States; Recruiting
Colorado
      University of Colorado Health Sciences Center, Denver,  Colorado,  80262,  United States; Not yet recruiting
District of Columbia
      The Washnigton Hospital Center, Washington,  District of Columbia,  20010,  United States; Recruiting
Georgia
      American Cardiology Research Institute, Atlanta,  Georgia,  30342,  United States; Recruiting
Illinois
      Rush University Medical Center, Chicago,  Illinois,  60612,  United States; Not yet recruiting
Indiana
      The Care Group at the Heart Center, Indianapolis,  Indiana,  46290,  United States; Recruiting
Louisiana
      Ochsner Clinic Foundation Metairie, Metairie,  Louisiana,  70002,  United States; Not yet recruiting
Maryland
      The Johns Hopkins Hospital, Baltimore,  Maryland,  21287,  United States; Recruiting
Massachusetts
      Caritas St. ELizabeth''''s Medical Center, CCP4C, Boston,  Massachusetts,  02135,  United States; Recruiting
      Yawkey Center for Outpatient Care, Boston,  Massachusetts,  02114,  United States; Not yet recruiting
Minnesota
      Minneapolis Heart Institute Foundation, Minneapolis,  Minnesota,  55415,  United States; Recruiting
Missouri
      Saint Louis University Hospital, St. Louis,  Missouri,  63104,  United States; Recruiting
New Hampshire
      Dartmouth-Hitchcock Medical Center, Lebanon,  New Hampshire,  03756,  United States; Recruiting
New York
      Mount Sinai School of Medicine, New York,  New York,  10029,  United States; Recruiting
      University of Rochester Medical Center, Rochester,  New York,  14642,  United States; Recruiting
      New York University School of Medicine, New York,  New York,  10003,  United States; Recruiting
North Carolina
      Duke University Medical Center, Durham,  North Carolina,  27705,  United States; Not yet recruiting
Ohio
      Jobst Vascular Center, Toledo,  Ohio,  43606,  United States; Recruiting
      Cleveland Clinic Foundation Desk S60, Cleveland,  Ohio,  44195,  United States; Recruiting
Oklahoma
      University of Oklahoma, Health Sciences Center, Oklahoma City,  Oklahoma,  73190,  United States; Not yet recruiting
Oregon
      Oregon Health & Science University, Portland,  Oregon,  97239,  United States; Not yet recruiting
Texas
      Baylor College of Medicine, Houston,  Texas,  77030,  United States; Recruiting
Germany
      Medizinische Klinik II Kardiologie and Pulmologie, Berlin,  12203,  Germany; Not yet recruiting
      Molekulare Kardiologie/Angiologie Medizinische Klinikund Poliklinik C, Munster,  48149,  Germany; Not yet recruiting
Germany, Karlsbad
      Klinikum Karlsbad Langensteinbach gGmbH, GuttmannstraBe 1,  Karlsbad,  76307,  Germany; Not yet recruiting
United Kingdom
      Hull Royal Infirmary, Hull,  HU3 2JZ,  United Kingdom; Not yet recruiting
      Derby City General Hospital, Derby,  DE22 3DT,  United Kingdom; Not yet recruiting
      Ninewells Hospital & Medical Center, Dundee,  DD1 9SY,  United Kingdom; Recruiting
      St. George''''s Hospital and Medical School, London,  SW17 0QT,  United Kingdom; Not yet recruiting
United Kingdom, Birmingham
      Selly Oak Hospital, Selly Oak,  Birmingham,  B29 6JD,  United Kingdom; Not yet recruiting
United Kingdom, Middlesex
      Ealing Hospital, Southall,  Middlesex,  UB1 3HW,  United Kingdom; Not yet recruiting
United Kingdom, Newcastle upon Tyre
      Freeman Hospital, Newcastle upon Tyne,  Newcastle upon Tyre,  NE7 7DN,  United Kingdom; Not yet recruiting
United Kingdom, Northern Ireland
      Belfast City Hospital, Belfast,  Northern Ireland,  BT9 7AB,  United Kingdom; Not yet recruiting

Study ID Numbers:  PADHIF00704
Record last reviewed:  June 2005
Last Updated:  July 25, 2005
Record first received:  July 7, 2005
ClinicalTrials.gov Identifier:  NCT00117650
Health Authority: United States: Food and Drug Administration
ClinicalTrials.gov processed this record on 2005-07-26