13C is a stable (i.e., non-radioactive) isotope of carbon with a natural abundance of 1%. Following infusion of [13C]glucose, in vivo MRS (magnetic resonance spectroscopy) can monitor the rate of flux of the 13C atom from glucose to glutamate to glutamine. Thus, this procedure can provide measure of glutamate (GLU) and glutamine (GLN) turnover in brain. We have established parameters to obtain these measurements in nonhuman primate brain. The current protocol seeks approval to optimize MRS parameters for human brain using the GE 3T device.

Further Study Details: 

Expected Total Enrollment:  30

(13)C is a stable (i.e., non-radioactive) isotope of carbon with a natural abundance of approximately 1%. Following infusion of [(13)C]glucose, in vivo MRS (magnetic resonance spectroscopy) can monitor the rate of flux of the (13)C atom from glucose to glutamate to glutamine. Thus, this procedure can provide measure of glutamate (GLU) and glutamine (GLN) turnover in brain. We have established parameters to obtain these measurements in nonhuman primate brain. The current protocol seeks approval to optimize MRS parameters for human brain using the GE 3T device.

Genders Eligible for Study:  Both

Criteria

INCLUSION CRITERIA:
Age: 18-65 years
Diagnosis: Healthy
EXCLUSION CRITERIA:
Serious medical illness (including diabetes) as determined from H&P or laboratory testing
All subjects must meet none of the Axis I diagnoses
Prescription psychotropic medication
Drug free period must be greater than 3 wk (anticholinergics and benzodiazepine) and greater than 8 wk (fluoxetine, antipsychotics, anticonvulsants)
Claustrophobia
Pregnancy
Women with child bearing potential will have pregnancy test to exclude pregnancy
Any condition that increases risk for MRI (e.g., pacemaker, metallic foreign body in the eye, etc.)
Unable to lay on one's back for MRI/MRS scans
Positive HIV test

Please refer to this study by ClinicalTrials.gov identifier  NCT00109174

Maryland
      National Institute of Mental Health (NIMH), 9000 Rockville Pike,  Bethesda,  Maryland,  20892,  United States; Recruiting

Study ID Numbers:  050144; 05-M-0144
Record last reviewed:  April 13, 2005
Last Updated:  April 30, 2005
Record first received:  April 22, 2005
ClinicalTrials.gov Identifier:  NCT00109174
Health Authority: United States: Federal Government
ClinicalTrials.gov processed this record on 2005-05-03