Laboratory Biosafety Publication

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systems to decontaminate or remove agents from exhaust air controlled access zones airlocks as laboratory entrances or separate buildings or modules to isolate the laboratory To review the CDC s comprehensive guidelines for each biosafety level a Web enabled edition of Biosafety in Microbiological and Biomedical Laboratories can be found at www cdc gov

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and design is critical to cage level containment and must be given serious consideration Safety requires protecting personnel animals and the environment around the clock While biosafety techniques and operational procedures used in concert with biocontainment equipment have proved effective in protecting personnel animals and environment they should

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decon systems Additional requirements outlined in CDC guidelines Because of these potential applications the integrity of the housing system must not be taken lightly The system must consistently and repeatedly provide a sealed environment

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conducted according to BSL 3 safety standards as outlined in the manual Biosafety in Microbiological and Biomedical Laboratories Health and Human Services publication No CDC 93 8395 Contact Doris J Bucher Ph D Associate Professor of Microbiology and Immunology

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Aliquoting of specimens in the existing biosafety level 2 lab facilities within the Virology Laboratory of ICDDR B

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security and animal exclusion zones Due to the varying risk of biological agents the facilities that handle these agents need to be designed and classified accordingly 1 Biosafety Laboratories There are four levels of biosafety laboratories which are designated in the United States as Levels 1 2 3 and 4 A Biosafety Level 1 or 2 BSL 1 or 2 facility

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